Human Podocalyxin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1658
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Virus
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot
Cited:
Cell Selection, ELISA Capture, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 222328
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Podocalyxin
Ser23-Arg427
Accession # AAB61574
Ser23-Arg427
Accession # AAB61574
Specificity
Detects human Podocalyxin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Podocalyxin or recombinant human Endoglycan is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Podocalyxin Antibody
Detection of Podocalyxin in BG01V Human Cells by Flow Cytometry.
BG01V human embryonic stem cells was stained with Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.Podocalyxin in BG01V Human Stem Cells.
Podocalyxin was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Detection of Podocalyxin in Induced pluripotent stem cells (iPSCs) by Flow Cytometry
Induced pluripotent stem cells (iPSCs) were stained with Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins.Applications for Human Podocalyxin Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: - BG01V human embryonic stem cells- Induced pluripotent stem cells (iPSCs)
Sample: - BG01V human embryonic stem cells- Induced pluripotent stem cells (iPSCs)
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells
Sample: Immersion fixed BG01V human embryonic stem cells
Western Blot
1 µg/mL
Sample: Recombinant Human Podocalyxin
Sample: Recombinant Human Podocalyxin
Reviewed Applications
Read 7 reviews rated 4.4 using MAB1658 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Podocalyxin
References
- Li, J. et al. (2001) DNA Seq. 12:407.
- Hara, T. et al. (1999) Immunity 11:567.
Alternate Names
GCTM, PCLP, POD XL, PODXL
Gene Symbol
PODXL
UniProt
Additional Podocalyxin Products
Product Documents for Human Podocalyxin Antibody
Product Specific Notices for Human Podocalyxin Antibody
For research use only
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