Human POMC Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9498
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 967905
Product Specifications
Immunogen
Human Adrenocorticotropic hormone/ACTH) synthetic peptide
Accession # P01189
Accession # P01189
Specificity
Detects human POMC in immunohistochemistry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human POMC Antibody
Detection of Human POMC by Western Blot.
Western blot shows lysates of human pituitary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human POMC Monoclonal Antibody (Catalog # MAB9498) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for POMC at approximately 10-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
POMC in Human Pituitary.
POMC was detected in immersion fixed paraffin-embedded sections of human pituitary using Mouse Anti-Human POMC Monoclonal Antibody (Catalog # MAB9498) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to corticotroph cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human POMC Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pituitary
Sample: Immersion fixed paraffin-embedded sections of human pituitary
Western Blot
0.5 µg/mL
Sample: Human pituitary gland tissue
Sample: Human pituitary gland tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: POMC
Long Name
Proopiomelanocortin
Alternate Names
ACTH, adrenocorticotropic hormone, adrenocorticotropin, alpha-melanocyte-stimulating hormone, alpha-MSH, beta-endorphin, beta-LPH, beta-melanocyte-stimulating hormone, beta-MSH, CLIP, corticotropin-like intermediary peptide, Corticotropin-lipotropin, gamma-LPH, gamma-MSH, lipotropin beta, lipotropin gamma, LPH, melanotropin alpha, melanotropin beta, melanotropin gamma, met-enkephalin, MSH, NPP, POC, pro-ACTH-endorphin, proopiomelanocortin, pro-opiomelanocortin, proopiomelanocortin preproprotein
Entrez Gene IDs
5443 (Human)
Gene Symbol
POMC
UniProt
Additional POMC Products
Product Documents for Human POMC Antibody
Product Specific Notices for Human POMC Antibody
For research use only
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