Human/Porcine/Canine CD90/Thy1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2067

R&D Systems, part of Bio-Techne
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AF2067
AF2067-SP
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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Canine

Cited:

Human, Porcine, Canine

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot

Cited:

Flow Cytomery, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all CD90/Thy1 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD90/Thy1
Gln20-Ser141
Accession # P04216

Specificity

Detects human CD90/Thy1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse CD90 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Porcine/Canine CD90/Thy1 Antibody

Detection of Human CD90/Thy1 antibody by Western Blot.

Detection of Human CD90/Thy1 by Western Blot.

Western blot shows lysates of human brain (cortex) tissue, human brain (hippocampus) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD90/Thy1 at approximately 22-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CD90/Thy1 antibody in BG01V Human Embyonic Stem Cells by Immunocytochemistry (ICC).

CD90/Thy1 in BG01V Human Embyonic Stem Cells.

CD90/Thy1 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
CD90/Thy1 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

CD90/Thy1 in Human Prostate Cancer Tissue.

CD90/Thy1 was detected in formalin fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Applications for Human/Porcine/Canine CD90/Thy1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µL/106 cells
Sample: Human mesenchymal stem cells, porcine mesenchymal stem cells, and canine mesenchymal stem cells

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells, canine mesenchymal stem cells and porcine mesenchymal stem cells

Immunohistochemistry

5-15 µg/mL
Sample: Formalin fixed paraffin-embedded sections of human prostate cancer tissue

Western Blot

1 µg/mL
Sample: Human brain (cortex) tissue, human brain (hippocampus) tissue, and human brain (hypothalamus) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using AF2067 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD90/Thy1

CD90 (also Thy1/Thymus cell antigen 1) is a 25-35 kDa glycoprotein member of the Immunoglobulin superfamily of molecules. It is expressed on neurons, ovarian follicular cells, endothelial cells, fibroblasts and circulating CD34+ stem cells, and appears to act as an adhesion molecule. CD90 is known to bind to integrins beta2, beta5 and beta3, the latter often accompanied by additional binding to syndecan-4. In the postnatal nervous system, this inhibits neurite outgrowth while stabilizing newly formed axonal networks. In the vascular system, CD90 mediates the extravasation of leukocytes. And in lung, a CD90: alphav beta5 interaction inhibits the extracellular activation of latent TGF-beta. Mature human CD90 is a 111 amino acid (aa) GPI-linked protein (aa 20-130). It contains one V-type Ig-like domain (aa 20-126) with a heparin-binding motif (aa 56-60), and a GPI-anchor amidated cysteine at position 130. The integrin binding site consists of an Arg35LeuAsp37 tripeptide. CD90 apparently forms 50‑60 kDa homodimers and 150 kDa homomultimers. There is one potential isoform variant that contains a 12 aa substitution for aa 1-13. Over aa 20-141, human CD90 shares 64% aa identity with mouse CD90 and 84% or 83% with horse and dog respectively.

Alternate Names

CD90, Thy1

Entrez Gene IDs

7070 (Human); 21838 (Mouse)

Gene Symbol

THY1

UniProt

P04216

Additional CD90/Thy1 Products

Product Documents for Human/Porcine/Canine CD90/Thy1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Porcine/Canine CD90/Thy1 Antibody

For research use only

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