Human POU2F3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11625
Key Product Details
| Species Reactivity: | Human |
| Immunogen: |
Synthetic Peptide Accession # Q9UKI9 |
| Applications: | Immunohistochemistry |
| Label: | Unconjugated |
| Source: | Monoclonal Mouse IgG2A Clone # 1091214 |
| Specificity: |
Detects a synthetic peptide specific for human POU2F3 around amino acid 100 in Direct ELISA. |
|
ImmunohistochemistryDetection of POU2F3 in Human Melanoma. POU2F3 was detected in immersion fixed paraffin-embedded sections of human melanoma using Mouse Anti-Human POU2F3 Monoclonal Antibody (Catalog # MAB11625) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
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