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Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1144G

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

FACS, Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 211309

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Pref-1/DLK1/FA1 long isoform
Ala24-Pro297 (with Arg248Pro and Lys295Ser substitutions)
Accession # P80370

Specificity

Detects human Pref‑1/DLK1/FA1 in direct ELISAs and Western blots. Shows approximately 50% cross-reactivity with recombinant mouse Pref‑1/DLK1/FA1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Antibody

Detection of Pref‑1/DLK1/FA1 in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1144G, filled histogram) or isotype control antibody (IC0041G, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Pref-1/DLK1/FA1

Pref-1 (Preadipocyte Factor 1), also known as DLK1 and FA1, is a 58-65 kDa member of the Notch/Serrata/Delta family of proteins.  It is expressed in prechondrocytes and preadipocytes and appears to block progenitor cell differentiation into mature cell lineages.  Mature human Pref-1 is a 360 amino acid (aa) type I transmembrane N- and O-linked glycoprotein.  It contains a 280 aa extracellular region (aa 24-303), a 24 aa transmembrane segment (aa 304-327), and a 56 aa cytoplasmic domain (aa 328-383).  The extracellular region contains six EGF-like domains, and undergoes proteolytic cleavage to generate a bioactive 50 kDa fragment, plus three 25-31 kDa fragments that show no activity.  There are multiple potential splice variants.  One shows a deletion of aa 229-301, a second possesses a six aa substitution for aa 1-52, a third shows a deletion of aa 210-277, while a fourth contains a six aa substitution for aa 207-383.  Over aa 24-297, human Pref-1 shares 82% aa identity with mouse Pref-1.

Long Name

Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1

Alternate Names

DLK-1, DLK1, FA1, pG2, Pref1, ZOG

Entrez Gene IDs

8788 (Human); 13386 (Mouse)

Gene Symbol

DLK1

UniProt

Additional Pref-1/DLK1/FA1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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