Human Pref-1/DLK1/FA1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1144
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 211309
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pref-1 long isoform
Ala24-Pro297 (with Arg248Pro and Lys295Ser substitutions)
Accession # P80370
Ala24-Pro297 (with Arg248Pro and Lys295Ser substitutions)
Accession # P80370
Specificity
Detects human Pref‑1/DLK‑1/FA1 in direct ELISAs and Western blots. Shows approximately 50% cross-reactivity with recombinant mouse Pref‑1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Pref-1/DLK1/FA1 Antibody
Detection of Pref-1/DLK1/FA1 in HepG2 Human Cell Line by Flow Cytometry.
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Pref-1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB1144, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Pref‑1/DLK1/FA1 in HepG2 Human Cell Line.
Pref-1/DLK1/FA1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Pref-1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB1144) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Pref-1/DLK1/FA1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Western Blot
1 µg/mL
Sample: Recombinant Human Pref‑1/DLK‑1/FA1 under non-reducing conditions only
Sample: Recombinant Human Pref‑1/DLK‑1/FA1 under non-reducing conditions only
Reviewed Applications
Read 4 reviews rated 4.8 using MAB1144 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pref-1/DLK1/FA1
Long Name
Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1
Alternate Names
DLK-1, DLK1, FA1, pG2, Pref1, ZOG
Gene Symbol
DLK1
UniProt
Additional Pref-1/DLK1/FA1 Products
Product Documents for Human Pref-1/DLK1/FA1 Antibody
Product Specific Notices for Human Pref-1/DLK1/FA1 Antibody
For research use only
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