Human Presenilin-1 N-Terminal Fragment Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB149
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 121121
Product Specifications
Immunogen
E. coli-derived recombinant human Presenilin‑1 N-Terminal Fragment
Lys1-Lys80
Accession # P49768
Lys1-Lys80
Accession # P49768
Specificity
Detects human Presenilin‑1 N-Terminal Fragment in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Presenilin‑2 N-Terminal Fragment, rhPresenilin‑1 C-Terminal Fragment, or rhPresenilin‑2 C-Terminal Fragment.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Presenilin-1 N-Terminal Fragment Antibody
Presenilin‑1 in Human Brain.
Presenilin-1 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Presenilin-1 N-Terminal Fragment Monoclonal Antibody (Catalog # MAB149) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Presenilin-1 N-Terminal Fragment Antibody
Application
Recommended Usage
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain and immersion fixed paraffin-embedded sections of human brain (medulla)
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain and immersion fixed paraffin-embedded sections of human brain (medulla)
Western Blot
1 µg/mL
Sample: Recombinant Human Presenilin-1 N-Terminal Fragment
Sample: Recombinant Human Presenilin-1 N-Terminal Fragment
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Presenilin-1
Presenilin‑1 (PS-1) is a multipass lysosomal membrane protein that is a component of the gamma secretase complex. It is cleaved by endoproteolysis to generate
N‑ and C‑terminal fragments. Some mutations in PS-1 are associated with the risk of early onset familial Alzheimer's disease.
Additional Presenilin-1 Products
Product Documents for Human Presenilin-1 N-Terminal Fragment Antibody
Product Specific Notices for Human Presenilin-1 N-Terminal Fragment Antibody
For research use only
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