Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3485
Key Product Details
Species Reactivity
Validated:
Human, Primate
Cited:
Human, Rat
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Simple Western, Western Blot
Cited:
ELISA Capture, ELISA Development, ELISA Development (Capture), ELISA Development (Detection), Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-like 4/ANGPLT4
Leu165-Ser406
Accession # Q9BY76
Leu165-Ser406
Accession # Q9BY76
Specificity
Detects human ANGPTL4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) ANGPTL3, rhAngiopoietin-1, -2, -3, and -4 is observed. In Western blots, approximately 1% cross-reactivity with rhANGPLT4 N-terminal Fragment is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antibody
Detection of Human/Primate Angiopoietin-like Protein 4/ANGPTL4 by Simple WesternTM.
Simple Western lane view shows lysates of Human Diabetic Adipose, loaded at 0.2 mg/mL. A specific band was detected for Angiopoietin-like Protein 4/ANGPTL4 at approximately 59 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3485). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antibody
Application
Recommended Usage
Simple Western
50 µg/mL
Sample: Human Diabetic Adipose
Sample: Human Diabetic Adipose
Western Blot
0.1 µg/mL
Sample: Recombinant Human Angiopoietin-like 4/ANGPTL4 (Catalog # 4487-AN)
Sample: Recombinant Human Angiopoietin-like 4/ANGPTL4 (Catalog # 4487-AN)
Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-like Protein 4/ANGPTL4
Alternate Names
Angiopoietin like Protein 4, ANGPTL4, FIAF, HFARP, PGAR
Gene Symbol
ANGPTL4
UniProt
Additional Angiopoietin-like Protein 4/ANGPTL4 Products
- All Products for Angiopoietin-like Protein 4/ANGPTL4
- Angiopoietin-like Protein 4/ANGPTL4 cDNA Clones
- Angiopoietin-like Protein 4/ANGPTL4 ELISA Kits
- Angiopoietin-like Protein 4/ANGPTL4 Lysates
- Angiopoietin-like Protein 4/ANGPTL4 Primary Antibodies
- Angiopoietin-like Protein 4/ANGPTL4 Proteins and Enzymes
- Angiopoietin-like Protein 4/ANGPTL4 Simple Plex
Product Documents for Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antibody
Product Specific Notices for Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antibody
For research use only
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