Human/Primate CCL5/RANTES Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB678
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Primate
Cited:
Human, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot
Cited:
Bioassay, ELISA Capture, ELISA Development, ELISA Microarray Development, Functional Assay, Immunocytochemistry, Luminex Development, Mass Spectometry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 21418
Product Specifications
Immunogen
E. coli-derived recombinant human CCL5/RANTES
Specificity
Detects human and primate CCL5/RANTES in direct ELISAs and Western blots. When used in combination with the biotinylated anti-human/primate CCL5/RANTES detection antibody (Catalog # BAF278) in sandwich ELISAs, no significant cross-reactivity or interference is observed with recombinant human (rh) ANG, rhCNTF, rh beta-ECGF, rhEGF, mouse (m) EGF, rhEpo, bovine (b) FGF acidic, rhFGF acidic, bFGF basic, rhFGF basic, rhFGF-4, rhG-CSF, rhGM-CSF, recombinant mouse (rm) GM-CSF, rhGRO alpha, rhIFN-gamma, rhIGF-I, rhIGF-II, rhIL-1 alpha, rmIL-1 alpha, rhIL-1 beta, rmIL‑1 beta, rhIL-1ra, recombinant rat (rr) IL-1ra, rhIL-2, rhIL-3, rmIL-3, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL‑10, rhIL-11, rhLIF, rhMCP‑1, rhM-CSF, rhMIP-1 alpha, rmMIP-1 alpha, rhMIP-1 beta, rmMIP-1 beta, rhOSM, human (h) PDGF, pPDGF, rhPDGF-AA, rhPDGF‑AB, rhPDGF-BB, rhSCF, rmSCF, rhSLPI, rhTGF-alpha, pTGF-beta 1, hTGF-beta 1, rhTGF-beta 1, pTGF-beta 2, rhTGF-beta 2, recombinant canine TGF-beta 3, rhTGF-beta 3, recombinant amphibian TGF-beta 5, rhTNF-alpha, rmTNF-alpha, or rmTNF-beta.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human/Primate CCL5/RANTES Antibody
Detection of Human CCL5/RANTES by Functional
The expression of chemokine CCL5 is up-regulated in co-cultures of BMMSCs cells and OTSCC.Co-culture of OTSCC cells with BMMSCs results in the high expression of chemokine CCL5 in HSC-3 and SAS cells, but not in dysplastic DOK cells (A). Function-blocking monoclonal antibody against CCL5 (Mab CCL5) slightly inhibits BMMSC enhanced invasion area, but has no effect on the invasion depth, whereas the antibody against CXCL1 does not increase the inhibitory effect in OTSCC invasion (B, C). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24204919), licensed under a CC-BY license. Not internally tested by R&D Systems.Detection of Human CCL5/RANTES by Immunocytochemistry/Immunofluorescence
RANTES filaments are present after exposure to shear stress.(A) HUVECs were electroporated with DNA encoding RANTES, cultivated on coverslips before stimulation with TNF alpha + IFN gamma for 30 h. RANTESwas labeled with an anti-RANTES antibody (clone 21418), and the cover slipswere mounted in a laminar flow chamber. The flow rate was adjusted to mimicvessel wall shear stress of 1 dyne/cm2. The imageswere acquired by confocal microscopy before (left image, 0 min) andafter 4 min with exposure to flow forces (right image, 4 min).(B) HUVECs were treated as described in (A) except that they were notlabeled with anti-RANTES antibody before exposure to flow. Human peripheralblood mononuclear cells were labeled with an anti-CD45 antibody, resuspendedin medium and applied by a pump to the flow chamber(1 dyne/cm2). After 10 min, the cells werelabeled with a rabbit anti-RANTES antibody. Images were acquired bysequential scanning confocal microscopy. Arrows and arrow heads indicateRANTES filaments and RANTES positive platelets, respectively. Originalmagnification in all panels, × 100. Scale bars, 10 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25791723), licensed under a CC-BY license. Not internally tested by R&D Systems.Neutralization by Human/Primate CCL5/RANTES Antibody.
Human/Primate CCL5/RANTES Antibody (Catalog # MAB678) neutralizes Recombinant Human CCL5/RANTES (278-RN) induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND50) for this effect is typically 80.0-800 ng/mL in the presence of 10 ng/mL Recombinant Human CCL5/RANTES.Applications for Human/Primate CCL5/RANTES Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human CCL5/RANTES (Catalog # 278-RN)
Sample: Recombinant Human CCL5/RANTES (Catalog # 278-RN)
Neutralization
Human/Primate CCL5/RANTES Antibody (Catalog # MAB678) neutralizes Recombinant Human CCL5/RANTES (Catalog # 278-RN) induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND50) for this effect is typically 80.0-800 ng/mL in the presence of 10 ng/mL Recombinant Human CCL5/RANTES.
Human/Primate CCL5/RANTES Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 4.5 using MAB678 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL5/RANTES
CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily. It binds and activates the chemokine receptors CCR1, 3 and 5.
Alternate Names
RANTES, SISd
Gene Symbol
CCL5
Additional CCL5/RANTES Products
Product Documents for Human/Primate CCL5/RANTES Antibody
Product Specific Notices for Human/Primate CCL5/RANTES Antibody
For research use only
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