Human/Primate MMP-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF513
Key Product Details
Species Reactivity
Validated:
Human, Primate
Cited:
Human, Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
ELISA Development, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-3
Tyr18-Cys477 (Lys45Glu)
Accession # P08254
Tyr18-Cys477 (Lys45Glu)
Accession # P08254
Specificity
Detects human and primate MMP-3 in ELISAs and Western blots. In sandwich ELISAs, less than 2.5% cross-reactivity with recombinant human(rh) MMP‑10 is observed and less than 0.1% cross-reactivity with rhMMP-1, -2, -7, -8, -9, -12, -13, and recombinant mouse MMP‑9 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Primate MMP-3 Antibody
Detection of Human MMP‑3 by Western Blot.
Western blot shows Recombinant Human MMP-3 Western Blot Standard Protein (Catalog # WBC015) and lysate of U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Primate MMP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-3 at approximately 57-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MMP‑3 in Human Bladder Cancer Tissue.
MMP-3 was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Goat Anti-Human/ Primate MMP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
MMP‑3 in Human Lung Cancer.
MMP-3 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Goat Anti-Human/Primate MMP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Primate MMP-3 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung cancer, human colon cancer tissue, and human bladder cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human lung cancer, human colon cancer tissue, and human bladder cancer tissue
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP-3 (Catalog # 513-MP), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP-3 (Catalog # 513-MP), see our available Western blot detection antibodies
Western Blot
2 µg/mL
Sample: Recombinant Human MMP-3 Western Blot Standard Proteins (Catalog # WBC015) and lysates of U‑118‑MG human glioblastoma/astrocytoma cell line
Sample: Recombinant Human MMP-3 Western Blot Standard Proteins (Catalog # WBC015) and lysates of U‑118‑MG human glioblastoma/astrocytoma cell line
Human/Primate MMP-3 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using AF513 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-3
Long Name
Matrix Metalloproteinase 3
Alternate Names
MMP3, Stromelysin 1
Gene Symbol
MMP3
UniProt
Additional MMP-3 Products
Product Documents for Human/Primate MMP-3 Antibody
Product Specific Notices for Human/Primate MMP-3 Antibody
For research use only
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