Recombinant Human MMP-3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 513-MP

R&D Systems, part of Bio-Techne
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513-MP-010
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Key Product Details

Source

NS0

Accession #

P08254

Structure / Form

Pro form

Conjugate

Unconjugated

Applications

Enzyme Activity

View all MMP-3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human MMP-3 protein
Tyr18-Cys477 (Lys45Glu)

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr18

Predicted Molecular Mass

52 kDa

SDS-PAGE

54-56 kDa doublet, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >150 pmol/min/µg, as measured under the described conditions.

Reviewed Applications

Read 3 reviews rated 5 using 513-MP in the following applications:

Formulation, Preparation and Storage

513-MP
Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl, CaCl2, Glycerol and Brij-35.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: MMP-3

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-3 (stromelysin-1), can degrade a broad range of substrates including collagen alpha chains, aggrecan, laminin, fibronectin, elastin, casein, alpha-1 antitrypsin, myelin basic protein, IL-1 beta, IGFBP-3, pro MMP-1, pro MMP-7, pro MMP-8, pro MMP-9 and pro MMP-13. MMP-3 does not cleave the triple helical region of interstitial collagens, a characteristic which distinguishes the stromelysins from the collagenases. The MMP-3 substrate repertoire extends beyond extracellular matrix proteins and implicates MMP-3 in roles other than direct tissue remodelling, for instance, enzyme cascades and cytokine regulation. MMP-3 is expressed by fibroblasts, chrondrocytes, osteoblasts, endothelial cells, smooth muscle cells and macrophages. Structurally, MMP-3 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

Long Name

Matrix Metalloproteinase 3

Alternate Names

MMP3, Stromelysin 1

Entrez Gene IDs

4314 (Human); 17392 (Mouse)

Gene Symbol

MMP3

UniProt

P08254

Additional MMP-3 Products

Product Documents for Recombinant Human MMP-3 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human MMP-3 Protein, CF

For research use only

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