Human PRKD3/nPKC nu Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6478
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PRKD3/nPKC nu
Ile465-Val559
Accession # O94806
Ile465-Val559
Accession # O94806
Specificity
Detects human PRKD3/nPKC nu in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human PRKD3/nPKC nu Antibody
Detection of Human PRKD3/nPKC nu by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human PRKD3/nPKC nu Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PRKD3/nPKC nu at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PRKD3/nPKC nu in MCF‑7 Human Cell Line.
PRKD3/nPKC nu was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human PRKD3/nPKC nu Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6478) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human PRKD3/nPKC nu Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line
Western Blot
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line
Sample: Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PRKD3/nPKC nu
Long Name
Protein Kinase D3
Alternate Names
EPK2, nPKC nu, PKC nu, PRKCN
Gene Symbol
PRKD3
UniProt
Additional PRKD3/nPKC nu Products
Product Documents for Human PRKD3/nPKC nu Antibody
Product Specific Notices for Human PRKD3/nPKC nu Antibody
For research use only
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