Human Pro‑BDNF Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB31751
Discontinued Product
MAB31751 has been discontinued.
View all BDNF products.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 584412
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Pro-BDNF
Ala19-Arg247
Accession # P23560
Ala19-Arg247
Accession # P23560
Specificity
Detects human Pro-BDNF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Pro-BDNF is observed and no cross-reactivity with recombinant human beta-NGF, NT-4, Pro-NT-3 or Pro-NT-4 is observed. In Western blots, 100% cross-reactivity with recombinant mouse Pro-BDNF is observed and no cross-reactivity with recombinant human mature BDNF or recombinant human NT-3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Pro‑BDNF Antibody
Detection of Human Pro‑BDNF by Western Blot.
Western blot shows lysates of human brain (cerebellum) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Pro-BDNF Monoclonal Antibody (Catalog # MAB31751) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Pro-BDNF at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of BDNF in Human Brain Cortex.
BDNF was detected in immersion fixed paraffin-embedded sections of human brain cortex using Mouse Anti-Human Pro BDNF Monoclonal Antibody (Catalog # MAB31751) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Pro‑BDNF Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain cortex
Sample: Immersion fixed paraffin-embedded sections of human brain cortex
Western Blot
2 µg/mL
Sample: Human brain (cerebellum) tissue
Sample: Human brain (cerebellum) tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BDNF
BDNF is a member of the NGF family of neurotrophic factors that are required for the differentiation and survival of neuronal subpopulations in the central and peripheral nervous systems. BDNF functions through interactions with the TrkB receptor tyrosine kinase and the low affinity neurotrophin receptor, p75 (NTR). The human BDNF cDNA encodes 247 amino acids (aa). Cleavage of an 18 aa signal sequence produces an approximately 35 kDa Pro-BDNF form. The N-terminal pro region of BDNF is removed by tPA and furin to release biologically active, 14 kDa BDNF. The propeptides of human and mouse BDNF share 93% aa sequence identity.
Long Name
Brain-derived Neurotrophic Factor
Alternate Names
Abrineurin, ANON2, BULN2, Neurotrophin
Gene Symbol
BDNF
UniProt
Additional BDNF Products
Product Documents for Human Pro‑BDNF Antibody
Product Specific Notices for Human Pro‑BDNF Antibody
For research use only
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