Human Pro Collagen I alpha1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6220
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio anubis (Olive Baboon), Xenograft
Applications
Validated:
Immunocytochemistry, Immunoprecipitation, Simple Western, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Collagen I
Gln23-Lys277, Gly1094-Leu1464
Accession # P02452
Gln23-Lys277, Gly1094-Leu1464
Accession # P02452
Specificity
Detects human Pro-Collagen I alpha 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human COL3A1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Pro Collagen I alpha1 Antibody
Detection of Human Pro-Collagen I alpha 1 by Western Blot.
Western blot shows lysates of human kidney tissue, human skin tissue, and human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro-Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pro-Collagen I alpha 1 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Detection of Human Collagen I by Western Blot.
Western blot shows lysates of IMR-90 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro Collagen I alpha1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Collagen I at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Pro-Collagen I alpha 1 in IMR‑90 Human Cell Line.
Pro-Collagen I alpha 1 was detected in immersion fixed IMR-90 human lung fibroblast cell line using Sheep Anti-Human Pro-Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Pro Collagen I alpha1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed IMR‑90 human lung fibroblast cell line
Sample: Immersion fixed IMR‑90 human lung fibroblast cell line
Immunoprecipitation
5 µg/mL
Sample: IMR‑90 human lung fibroblast cell line
Sample: IMR‑90 human lung fibroblast cell line
Simple Western
10 µg/mL
Sample: IMR-90 human lung fibroblast cell line
Sample: IMR-90 human lung fibroblast cell line
Western Blot
1 µg/mL
Sample: Human kidney tissue, human skin tissue, human cartilage tissue, and IMR‑90 human lung fibroblast cell line
Sample: Human kidney tissue, human skin tissue, human cartilage tissue, and IMR‑90 human lung fibroblast cell line
Reviewed Applications
Read 1 review rated 5 using AF6220 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collagen I alpha 1
References
- Canty, E.G. et al. (2005) J. Cell Sci. 118:1341.
- Han, S. et al. (2008) J. Mol. Biol. 383:122.
Alternate Names
COL1A1, OI4
Entrez Gene IDs
1277 (Human)
Gene Symbol
COL1A1
UniProt
Additional Collagen I alpha 1 Products
Product Documents for Human Pro Collagen I alpha1 Antibody
Product Specific Notices for Human Pro Collagen I alpha1 Antibody
For research use only
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