Human Pro MMP-13 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB913
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Porcine
Applications
Validated:
ELISA, Immunoaffinity Purification, Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 87518
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-13
Leu20-Cys471
Accession # P45452
Leu20-Cys471
Accession # P45452
Specificity
Detects human Pro MMP‑13 in direct ELISAs and Western blots. Does not detect active recombinant human MMP-13. In Western blots, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, or -12 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Pro MMP-13 Antibody
Human Pro- MMP‑13 ELISA Standard Curve.
Recombinant Human Pro-MMP-13 protein was serially diluted 2-fold and captured by Mouse Anti-Human MMP-13 Monoclonal Antibody (Catalog # MAB5111) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Pro MMP-13 Monoclonal Antibody (Catalog # MAB913) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Pro MMP-13 Antibody
Application
Recommended Usage
ELISA
This antibody functions as a Pro-MMP-13 ELISA ELISA detection antibody when paired with Mouse Anti-Human MMP‑13 Monoclonal Antibody (Catalog # MAB5111).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Pro-MMP-13 DuoSet ELISA Kit (Catalog # DY913) for convenient development of a sandwich ELISA or the Human Pro-MMP-13 Quantikine ELISA Kit (Catalog # DM1300) for a complete optimized ELISA.
Immunoaffinity Purification
Sepharose beads coupled with this antibody have been used to immunoprecipitate and immunopurify Recombinant Human Pro-MMP-13 (Catalog # 511-MM) from condition media.
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑13 (Catalog # 511‑MM), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑13 (Catalog # 511‑MM), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human MMP-13 Western Blot Standard (Catalog # WBC020) under non‑reducing conditions only
Sample: Recombinant Human MMP-13 Western Blot Standard (Catalog # WBC020) under non‑reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB913 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-13
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.
References
- Jeffery, J.J. (1998) in Collagenase 3. A.J. Barrett, et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1167.
Long Name
Matrix Metalloproteinase 13
Alternate Names
MMP13
Entrez Gene IDs
4322 (Human)
Gene Symbol
MMP13
UniProt
Additional MMP-13 Products
Product Documents for Human Pro MMP-13 Antibody
Product Specific Notices for Human Pro MMP-13 Antibody
For research use only
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