Human Progesterone R/NR3C3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5415
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot
Cited:
Functional Assay
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PR-B
Met1-Leu189
Accession # P06401
Met1-Leu189
Accession # P06401
Specificity
Detects human PR-A and PR-B in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Progesterone R/NR3C3 Antibody
Detection of Human Progesterone R/NR3C3 by Western Blot.
Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PR-A and PR-B at approximately 90 and 118 kDa, respectively (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Progesterone R/NR3C3 in T47D Human Cell Line.
Progesterone R/NR3C3 was detected in immersion fixed T47D human breast cancer cell line using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Progesterone R/NR3C3 in Human Prostate Cancer Tissue.
Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Progesterone R/NR3C3 Antibody
Application
Recommended Usage
Immunocytochemistry
1-15 µg/mL
Sample: Immersion fixed T47D human breast cancer cell line
Sample: Immersion fixed T47D human breast cancer cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue
Simple Western
25 µg/mL
Sample: T47D human breast cancer cell line
Sample: T47D human breast cancer cell line
Western Blot
1 µg/mL
Sample: T47D human breast cancer cell line
Sample: T47D human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Progesterone R/NR3C3
Progesterone Receptor B (PR-B) is a 118 kDa member of the NR3 subfamily within the nuclear hormone receptor family of proteins. It is expressed in female reproductive tissues as well as neurons throughout the CNS. PR-B is particularly important in the mammary gland where it mediates proliferative responses to progesterone. Human PR-B is 933 amino acids (aa) in length. It contains an N-terminal regulatory region (aa 1‑566), a DNA binding domain (aa 567‑639), and a
steroid-binding region (aa 681‑933). Ligand binding induces a key phosphorylation event at Ser294 by ERK1/2. An alternate start site at Met165 generates 90 kDa
PR‑A, an isoform particularly important in the ovary and uterus that insures fertility. Other isoforms show a deletion of either aa 637‑738 or 598‑636, or a 17 aa substitution for aa 787‑933.
Long Name
Progesterone Receptor
Alternate Names
NR3C3, PGR, ProgesteroneR
Entrez Gene IDs
5241 (Human)
Gene Symbol
PGR
UniProt
Additional Progesterone R/NR3C3 Products
Product Documents for Human Progesterone R/NR3C3 Antibody
Product Specific Notices for Human Progesterone R/NR3C3 Antibody
For research use only
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