A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Protocadherin-1 Monoclonal Antibody (Catalog # MAB5899, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Protocadherin‑1 in BG01V Human Embryonic Stem Cells.

Protocadherin-1 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human Protocadherin-1 Monoclonal Antibody (Catalog # MAB5899) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Protocadherin-1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: A431 human epithelial carcinoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protocadherin-1

Protocadherin-1 (PCDH-1; also PC42) is a 150 - 170 kDa delta1 subgroup member of the nonclustered protocadherin family of molecules. It is expressed on macrophages, respiratory epithelium, endothelial cells and neurons. Protocadherin-1 apparently forms homophilic Ca⁺⁺-dependent complexes and likely serves as an adhesion molecule. Human full-length Protocadherin-1 precursor is 1237 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 795 aa extracellular domain (ECD) (aa 58-852) plus a 364 aa cytoplasmic region. There are seven cadherin domains in the ECD (aa 58-844), and an RRVTF cytoplasmic motif that binds PP1 alpha phosphatase. There are multiple splice variants. Alternative start sites exist at Met23 and Met235, there is a deletion of aa 209-220, and a 27 aa substitution for aa 1034-1237. Over aa 58-162, human Protocadherin-1 shares 96% aa identity with mouse Protocadherin-1.

Alternate Names

PC42, PCDH1, PCDH42, Protocadherin1

Entrez Gene IDs

5097 (Human); 75599 (Mouse); 307481 (Rat)

Gene Symbol

PCDH1

UniProt

Q08174

Additional Protocadherin-1 Products

Product Documents for Human Protocadherin-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Protocadherin-1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Protocadherin-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5899

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all Protocadherin-1 Antibodies »

Product Summary for Human Protocadherin-1 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Protocadherin-1 Antibody

Detection of Protocadherin‑1 in A431 Human Cell Line by Flow Cytometry.