Human Protocadherin-8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5434

R&D Systems, part of Bio-Techne
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MAB5434
MAB5434-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 562615

View all Protocadherin-8 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Protocadherin-8
Lys30-Thr479
Accession # O95206

Specificity

Detects human Protocadherin-8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Protocadherin-1, -10, or -15 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Protocadherin-8 Antibody

Protocadherin-8 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

Protocadherin‑8 in MCF‑7 Human Cell Line.

Protocadherin-8 was detected in immersion fixed MCF-7 human breast cancer cell line using Human Protocadherin-8 Monoclonal Antibody (Catalog # MAB5434) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Protocadherin-8 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protocadherin-8

Protocadherin 8 (PCDH-8) is a 160 kDa group F member of the protocadherin family of molecules.  It is expressed in breast epithelium, the synaptic membranes of neurons, and presumably forms Ca++-dependent homophilic complexes.  In breast, it is believed to contribute to a stable epithelial architecture, while in brain, it impacts LTP.  Mature human PCDH-8 is a type I transmembrane protein that is 1041 amino acids (aa) in length.  It contains a 720aa extracellular domain (ECD) (aa 30-749) plus a 300 aa cytoplasmic region.  There are six cadherin domains in the ECD (aa 30-723).  Multiple splice variants exist.  Two show deletions of aa 134-607 and 781-877, respectively, while a third shows a six aa substitution for aa 365-527.  Over aa 30-689, human PCDH-8 shares 93% aa identity with mouse PCDH-8.

Alternate Names

Arcadlin, PAPC, PCDH8, Protocadherin8

Entrez Gene IDs

5100 (Human); 18530 (Mouse); 64865 (Rat)

Gene Symbol

PCDH8

UniProt

O95206

Additional Protocadherin-8 Products

Product Documents for Human Protocadherin-8 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Protocadherin-8 Antibody

For research use only

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