Human PSENEN Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6859
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 665521
Product Specifications
Immunogen
Peptide containing human PSENEN
Gly89-Gly98
Accession # Q9NZ42
Gly89-Gly98
Accession # Q9NZ42
Specificity
Detects human PSENEN in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human PSENEN Antibody
PSENEN in Human Brain.
PSENEN was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Human PSENEN Monoclonal Antibody (Catalog # MAB6859) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and neurites of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human PSENEN Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PSENEN
Gamma-secretase subunit PSENEN, also called PEN-2 (Presenilin enhancer protein 2) is a 10 kDa member of the PEN-2 family. Human PSENEN is 101 amino acids (aa) in length, and is a multi-pass transmembrane protein. Residues 1-17 are on the luminal side of the endoplasmic reticulum or Golgi apparatus, where PSENEN is primarily located. Amino acids 18-38 form a transmembrane region, and aa 39-60 form a cytoplasmic segment. Another transmembrane segment is formed by residues 61-81, and residues 82-101 are located on the luminal side of the ER or cis-Golgi. Human PSENEN shares 96% aa sequence identity with mouse and rat PSENEN. Functionally, PSENEN is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and beta-amyloid precursor protein.
Long Name
Presenilin Enhancer 2 Homolog
Alternate Names
PEN-2
Gene Symbol
PSENEN
UniProt
Additional PSENEN Products
Product Documents for Human PSENEN Antibody
Product Specific Notices for Human PSENEN Antibody
For research use only
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