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Human PSG6 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8598

R&D Systems, part of Bio-Techne
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MAB8598-100
MAB8598-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 935926

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived transfected with human PSG6
Met1-Gly424
Accession # Q00889

Specificity

Detects human PSG6 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human PSG6 Antibody

Detection of Human PSG6 antibody by Western Blot.

Detection of Human PSG6 by Western Blot.

Western blot shows human serum from a pregnant donor (+) compared to a non-pregnant donor (-). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PSG6 Monoclonal Antibody (Catalog # MAB8598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PSG6 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PSG6 antibody in Human Placenta by Immunohistochemistry (IHC-P).

PSG6 in Human Placenta.

PSG6 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human PSG6 Monoclonal Antibody (Catalog # MAB8598) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human PSG6 Antibody

Application
Recommended Usage

Immunohistochemistry

5 µg/mL
Sample: Human placenta tissue

Western Blot

0.5 µg/mL
Sample: Human serum from a pregnant donor

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PSG6

Pregnancy-specific beta-1-glycoprotein 6 is encoded by the PSG6 gene. High levels of PSG biosynthesis are restricted to the placenta syncytiotrophoblast and are essential for the maintenance of normal gestation in mammalian species.

Long Name

Pregnancy-specific beta-1-glycoprotein 6

Alternate Names

PSBG-10, PSBG-12, PSG-6, PSGGB

Entrez Gene IDs

5675 (Human)

Gene Symbol

PSG6

UniProt

Additional PSG6 Products

Product Documents for Human PSG6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PSG6 Antibody

For research use only

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