Human PSMA/FOLH1/NAALADase I Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4234
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Cited:
Capture Assay, ELISA Capture
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human PSMA/FOLH1/NAALADase I
Lys44-Ala750
Accession # Q04609
Lys44-Ala750
Accession # Q04609
Specificity
Detects human PSMA/FOLH1/NAALADase I in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant mouse NAALADase II is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human PSMA/FOLH1/NAALADase I Antibody
Detection of Human PSMA/FOLH1/ NAALADase I by Western Blot.
Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human PSMA/FOLH1/ NAALADase I Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF4234) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PSMA/FOLH1/NAALADase I at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of PSMA/FOLH1/ NAALADase I in LnCAP Human Cell Line by Flow Cytometry.
LnCAP human prostate cancer cell line was stained with Human PSMA/FOLH1/NAALADase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4234, filled histo-gram) or control antibody (Catalog # 5-001-A, open histo-gram), followed by Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
PSMA/FOLH1/NAALADase I in Human Prostate Cancer Tissue.
PSMA/FOLH1/NAALADase I was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human PSMA/FOLH1/NAALADase I Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF4234) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and extracellular space. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human PSMA/FOLH1/NAALADase I Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human PSMA/FOLH1/NAALADase I Monoclonal Antibody (Catalog # MAB42341).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human PSMA/FOLH1 DuoSet ELISA Kit (Catalog # DY4234-05) for convenient development of a sandwich ELISA.
Flow Cytometry
2.5 µg/106 cells
Sample: LnCAP human prostate cancer cell line
Sample: LnCAP human prostate cancer cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue
Western Blot
0.2 µg/mL
Sample: Human prostate tissue
Sample: Human prostate tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PSMA/FOLH1/NAALADase I
Human prostate-specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1, 2). The enzyme is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, folypoly-gamma-glutamate carboxypeptidase (FGCP), and N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity, such as stroke, chronic pain, and amyotrophic lateral sclerosis (3). Intestinal PSMA hydrolyzes folylpoly-gamma -glutamates, facilitating the uptake of folate (4).
References
- Silver, D.A. et al. (1997) Clin. Cancer Res. 3:81.
- Carter, R.E. et al. (1996) Pro. Natl. Acad. Sci. USA 93:749.
- Jackson, P.F. and B.S. Slusher (2001) Curr. Med. Chem. 8:949.
- Heston, W.D. (1997) Urology 49:104.
Long Name
Prostate-specific Membrane Antigen
Alternate Names
FGCP, FOLH1, GCP2, GCPII, mopsm, NAALAD1, NAALADase I
Gene Symbol
FOLH1
UniProt
Additional PSMA/FOLH1/NAALADase I Products
Product Documents for Human PSMA/FOLH1/NAALADase I Antibody
Product Specific Notices for Human PSMA/FOLH1/NAALADase I Antibody
For research use only
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