Human PU.1/Spi-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5870
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PU.1
Met1-Lys169
Accession # NP_001074016
Met1-Lys169
Accession # NP_001074016
Specificity
Detects human PU.1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human SPI-B is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human PU.1/Spi-1 Antibody
Detection of Human PU.1 by Western Blot.
Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PU.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5870) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PU.1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
PU.1 in THP‑1 Human Cell Line.
PU.1 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Human PU.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5870) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human PU.1/Spi-1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed THP-1 human acute monocytic leukemia cell line
Sample: Immersion fixed THP-1 human acute monocytic leukemia cell line
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: PMA and Ca2+ ionomycin‑treated human Th2-stimulated PBMCs, fixed with paraformaldehyde, and permeabilized with saponin
Sample: PMA and Ca2+ ionomycin‑treated human Th2-stimulated PBMCs, fixed with paraformaldehyde, and permeabilized with saponin
Western Blot
1 µg/mL
Sample: THP-1 human acute monocytic leukemia cell line
Sample: THP-1 human acute monocytic leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PU.1/Spi-1
Long Name
Hematopoietic transcription factor PU.1
Alternate Names
OF, SFPI1, Spi-1, SPI-A, SPI1
Gene Symbol
SPI1
UniProt
Additional PU.1/Spi-1 Products
Product Documents for Human PU.1/Spi-1 Antibody
Product Specific Notices for Human PU.1/Spi-1 Antibody
For research use only
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