HEK293 human embryonic kidney cell line transfected with either (A) human PVRIG or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human PVRIG Monoclonal Antibody (Catalog # MAB93652) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Nectin-2 Binding to PVRIG Blocked by Human PVRIG Antibody.

In a functional ELISA, Recombinant Human Nectin-2/CD112 (Catalog # 2229-N2) binds to immobilized Recombinant Human PVRIG Fc Chimera (Catalog # 9365-PV) coated at 1 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Rabbit Anti-Human PVRIG Monoclonal Antibody (Catalog # MAB93652). At 2.5 µg/mL, this antibody will block >90% of the binding.

Applications for Human PVRIG Antibody

Application

Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 40-240 Ng/mL of this antibody will block 50% of the binding of 2.5 μg/mL of Recombinant Human Nectin-2/CD112 (Catalog # 2229-N2) to immobilized Recombinant Human PVRIG Fc Chimera (Catalog # 9365-PV) coated at 1 µg/mL (100 µL/well). At 2.5 µg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human PVRIG and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PVRIG

Human PVRIG (Poliovirus Receptor Related Immunoglobulin Domain-containing Protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family (1). It is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. The intracellular domain contains two tyrosine residues, one within an ITIM-like motif that is a potential docking site for phosphatases (1). The extracellular domain sequence of human and mouse PVRIG have approximately 65% similarity. The human PVRIG gene is preferentially expressed in lymphocytes, such as T cells and NK cells, but not in monocyte derived dendritic cells (1). PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. Disrupting the PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is a novel checkpoint for human T cells (1).

References

Zhu, Y., et.al. (2016) J. Exp. Med. 213:167.

Long Name

Poliovirus Receptor Related Immunoglobulin Domain Containing

Alternate Names

C7orf15, CD112R

Entrez Gene IDs

79037 (Human); 102640920 (Mouse); 102127364 (Cynomolgus Monkey)

Gene Symbol

PVRIG

UniProt

Q6DK17

Additional PVRIG Products

Product Documents for Human PVRIG Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human PVRIG Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human PVRIG Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB93652

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all PVRIG Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human PVRIG Antibody

Detection of PVRIG in HEK293 Human Cell Line Transfected with Human PVRIG and eGFP by Flow Cytometry.