Human Pygopus-2 Antibody

Detection of Human Pygopus‑2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human Pygopus-2 Monoclonal Antibody (Catalog # MAB3616) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pygopus-2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Pygopus‑2 in MCF‑7 Human Cell Line.

Pygopus-2 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Pygopus-2 Monoclonal Antibody (Catalog # MAB3616) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Pygopus‑2 by Simple Western^TM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Pygopus‑2 at approximately 65 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Pygopus‑2 Monoclonal Antibody (Catalog # MAB3616). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.