Human/Rat CCR8 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1429A

Clone 191704 was used by HLDA to establish CD designation
R&D Systems, part of Bio-Techne
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FAB1429A-100
FAB1429A-025
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 191704

View all CCR8 Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human CCR8
Met1-Leu355
Accession # P51685

Specificity

Detects human and rat CCR8. Neutralizes the bioactivity of human CCR8 and specifically stains human CCR8-transfected cell lines but not the parent cell lines.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Rat CCR8 APC-conjugated Antibody

Detection of CCR8 antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of CCR8 in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Rat Anti-Human/Rat CCR8 APC-conjugated Monoclonal Antibody (Catalog # FAB1429A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR8 antibody in Rat Peripheral Blood Cells antibody by Flow Cytometry.

Detection of CCR8 in Rat Peripheral Blood Cells by Flow Cytometry.

Rat peripheral blood cells were stained with Rat Anti-Human/Rat CCR8 APC-conjugated Monoclonal Antibody (Catalog # FAB1429A) and Anti-Rat CD3 PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Rat CCR8 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood monocytes and rat peripheral blood cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.7 using FAB1429A in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied in a saline solution containing BSA and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR8

CCR8 (C-C chemokine receptor 8; previously called CKR-L1 or TER1 and designated CD198) is a 41-43 kDa member of the GPCR 1 family of transmembrane proteins.  CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons.  CCL1/TCA3 and vMIP-1 are known agonists for CCR8.  Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) in length.  It contains a 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105 collectively, human and mouse CCR8 share 64% aa sequence identity.

Alternate Names

CCR8, CD198, CDw198, ChemR1, CKRL1, GPRCL1, TER-1

Entrez Gene IDs

1237 (Human); 12776 (Mouse)

Gene Symbol

CCR8

UniProt

P51685

Additional CCR8 Products

Product Documents for Human/Rat CCR8 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Rat CCR8 APC-conjugated Antibody

For research use only

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