Human/Rat Laminin gamma1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2139
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Rat
Applications
Validated:
Immunocytochemistry, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # D18
Product Specifications
Immunogen
Rat glomerular basement membrane
Specificity
Detects human and rat Laminin gamma1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Rat Laminin gamma1 Antibody
Laminin gamma1 in LS180 Human Cell Line.
Laminin gamma1 was detected in immersion fixed LS180 human colorectal adenocarcinoma cell line using Mouse Anti-Human/Rat Laminin gamma1 Monoclonal Antibody (Catalog # MAB2139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Rat Laminin gamma1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed LS180 human colorectal adenocarcinoma cell line and immersion fixed HepG2 human hepatocellular carcinoma cell line
Sample: Immersion fixed LS180 human colorectal adenocarcinoma cell line and immersion fixed HepG2 human hepatocellular carcinoma cell line
Immunoprecipitation
Green, T.L. et al. (1992) J. Biol. Chem. 267:2014.
Western Blot
Green, T.L. et al. (1992) J. Biol. Chem. 267:2014. This application was not tested by R&D Systems.
Reviewed Applications
Read 3 reviews rated 3.7 using MAB2139 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Laminin gamma 1
References
- Colognato, H. et al. (2000) Dev. Dyn. 218:213.
Alternate Names
LAMC1, Laminin B2
Gene Symbol
LAMC1
Additional Laminin gamma 1 Products
Product Documents for Human/Rat Laminin gamma1 Antibody
Product Specific Notices for Human/Rat Laminin gamma1 Antibody
For research use only
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