Human/Rat Neurogenin-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3314
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 7G4
Product Specifications
Immunogen
E. coli-derived recombinant rat Neurogenin-2
Specificity
Detect human and rat Neurogenin‑2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Rat Neurogenin-2 Antibody
Detection of Neurogenin-2 in Human Cerebellum.
Neurogenin-2 was detected in immersion fixed paraffin-embedded sections of human cerebellum using Mouse Anti-Human/Rat Neurogenin-2 Monoclonal Antibody (Catalog # MAB3314) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Neurogenin-2 in Rat Brain.
Neurogenin-2 was detected in immersion fixed paraffin-embedded sections of rat brain using Mouse Anti-Human/Rat Neurogenin-2 Monoclonal Antibody (Catalog # MAB3314) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Rat Neurogenin-2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human cerebellum and rat brain
Sample: Immersion fixed paraffin-embedded sections of human cerebellum and rat brain
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neurogenin-2
Alternate Names
Atoh4, Math4A, NEUROG2, Neurogenin2
Gene Symbol
NEUROG2
Additional Neurogenin-2 Products
Product Documents for Human/Rat Neurogenin-2 Antibody
Product Specific Notices for Human/Rat Neurogenin-2 Antibody
For research use only
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