OV-6 was detected in immersion fixed BG01V human embryonic stem cells differentiated into hepatocytes using Mouse Anti-Human/Rat OV-6 Monoclonal Antibody (Catalog # MAB2020) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human/Rat OV-6 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

Parent, R. et al. (2004) Gastroenterology 126(4):1147.
He, Z.P. et al. (2003) Cell Prolif. 37(2):177.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cell bodies and BG01V human embryonic stem cells differentiated into hepatocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OV-6

OV-6 antigen has been used as a marker to identify oval cells, putative hepatic stem cells that can differentiate into multiple cell types.

References

Dunsford, H.A. and S. Sell (1989) Cancer Res. 49:4887.
Parent, R. et al. (2004) Gastroenterology 126:1147.
He, Z.P. et al. (2003) Cell Prolif. 37:177.
Lazaro, C.A. et al. (2003) Hepatology 38:1095.
He, Z.P. et al. (2003) Differentation 71:281.

Long Name

Oval Cell Marker

Alternate Names

OV6

Additional OV-6 Products

Product Documents for Human/Rat OV-6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Rat OV-6 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human/Rat OV-6 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2020

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all OV-6 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Rat OV-6 Antibody

OV‑6 in BG01V Human Embryonic Stem Cells.