Human/Rat PGD2 Synthase/PTGDS Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10099
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 997303
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PGD2 Synthase/PTGDS
Met1-Gln190
Accession # P41222
Met1-Gln190
Accession # P41222
Specificity
Detects human and rat PGD2 Synthase/PTGDS in direct ELISA and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Rat PGD2 Synthase/PTGDS Antibody
Detection of Human and Rat PGD2 Synthase/PTGDS by Western Blot.
Western blot shows lysates of human heart tissue and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Rat PGD2 Synthase/PTGDS Monoclonal Antibody (Catalog # MAB10099) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PGD2 Synthase/PTGDS at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PGD2 Synthase/PTGDS in Human Brain.
PGD2 Synthase/PTGDS was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human/Rat PGD2 Synthase/PTGDS Monoclonal Antibody (Catalog # MAB10099) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Rat PGD2 Synthase/PTGDS Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Western Blot
0.5 µg/mL
Sample: Human heart tissue and Rat brain tissue
Sample: Human heart tissue and Rat brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PGD2 Synthase/PTGDS
Long Name
Prostaglandin D2 Synthase
Alternate Names
Cerebrin-28, PDS, PGD2, PGDS2, PH2DISO, PTGDS
Gene Symbol
PTGDS
UniProt
Additional PGD2 Synthase/PTGDS Products
Product Documents for Human/Rat PGD2 Synthase/PTGDS Antibody
Product Specific Notices for Human/Rat PGD2 Synthase/PTGDS Antibody
For research use only
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