HEK293 human embryonic kidney cell line either (A) transfected with human Reduced Folate Carrier/SLC19A1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Reduced Folate Carrier/SLC19A1 Monoclonal Antibody (Catalog # MAB8450) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).

Applications for Human Reduced Folate Carrier/SLC19A1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human Reduced Folate Carrier/SLC19A1 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reduced Folate Carrier/SLC19A1

SLC19A1, also known as, Reduced Folate Carriers, is a transmembrane transporter that is involved in the regulation of intracellular concentrations of folate. There is an association between idiopathic recurrent spontaneous abortion (RSA) and 3 SLC19A1 polymorphisms that may be useful biomarkers for RSA risk. Three transcript variants encoding different isoforms have been found for this gene.

Long Name

Solute Carrier Family 12 (Folate Transporter) Member 3

Alternate Names

CHMD, FOLT, IFC1, REFC, RFC1

Entrez Gene IDs

6573 (Human); 20509 (Mouse); 29723 (Rat)

Gene Symbol

SLC19A1

UniProt

P41440

Additional Reduced Folate Carrier/SLC19A1 Products

Product Documents for Human Reduced Folate Carrier/SLC19A1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Reduced Folate Carrier/SLC19A1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Reduced Folate Carrier/SLC19A1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8450

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Reduced Folate Carrier/SLC19A1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Reduced Folate Carrier/SLC19A1 Antibody

Detection of Reduced Folate Carrier/SLC19A1 in HEK293 Human Cell Line Transfected with Human Reduced Folate Carrier/SLC19A1 and eGFP by Flow Cytometry.