Human Reg3A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5965
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 512124
Product Specifications
Immunogen
E. coli-derived recombinant human Reg3A
Glu27-Asp175
Accession # Q06141
Glu27-Asp175
Accession # Q06141
Specificity
Detects human Reg3A in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Reg3A Antibody
Detection of Human Reg3A by Western Blot.
Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Reg3A Monoclonal Antibody (Catalog # MAB5965) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Reg3A at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reg3A in HepG2 Human Cell Line.
Reg3A was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Reg3A Mono-clonal Antibody (Catalog # MAB5965) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Reg3A Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed Huh-7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line
Sample: Immersion fixed Huh-7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line
Western Blot
2 µg/mL
Sample: Human small intestine tissue
Sample: Human small intestine tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Reg3A
Long Name
Regenerating Islet-derived IIIa
Alternate Names
Lithostathine 3, PAP2, REG3A
Gene Symbol
REG3A
UniProt
Additional Reg3A Products
Product Documents for Human Reg3A Antibody
Product Specific Notices for Human Reg3A Antibody
For research use only
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