Human RelB Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2698
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 315206
Product Specifications
Immunogen
E. coli-derived recombinant human RelB
Gln382-Thr579
Accession # Q01201
Gln382-Thr579
Accession # Q01201
Specificity
Detects human RelB.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human RelB Antibody
RelB in Human Lymphoma.
RelB was detected in immersion fixed paraffin-embedded sections of human lymphoma using 8 µg/mL Mouse Anti-Human RelB Monoclonal Antibody (Catalog # MAB2698) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human RelB by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human RelB Monoclonal Antibody (Catalog # MAB2698) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RelB at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
RelB in Raji Human Cell Line.
RelB was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Mouse Anti-Human RelB Monoclonal Antibody (Catalog # MAB2698) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human RelB Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and Raji human Burkitt's lymphoma cell line
Sample: Immersion fixed human peripheral blood mononuclear cells and Raji human Burkitt's lymphoma cell line
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymphoma
Sample: Immersion fixed paraffin-embedded sections of human lymphoma
Western Blot
0.1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line
Sample: Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line
Reviewed Applications
Read 3 reviews rated 3.7 using MAB2698 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RelB
Long Name
v-rel Reticuloendotheliosis Viral Oncogene Homolog B
Alternate Names
IREL
Entrez Gene IDs
5971 (Human)
Gene Symbol
RELB
UniProt
Additional RelB Products
Product Documents for Human RelB Antibody
Product Specific Notices for Human RelB Antibody
For research use only
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