Human ROBO4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2454

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
MAB2454
MAB2454-SP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 265703

View all ROBO4 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ROBO4
Gln28-Arg467
Accession # Q8WZ75

Specificity

Detects human ROBO4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant rat ROBO1, recombinant human (rh) ROBO2, or rhROBO3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human ROBO4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells

Western Blot

1 µg/mL
Sample: Recombinant Human ROBO4

Reviewed Applications

Read 2 reviews rated 3.5 using MAB2454 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROBO4

ROBO4, also called magic roundabout, is a ~150 kDa glycoprotein belonging to the ROBO family (1). ROBOs are molecular guidance receptors that typically interact with Slit ligands to regulate axon guidance and neuronal migration (2). Unlike other family members, ROBO4 is mainly restricted to the vascular endothelium (1, 2). Expression in early hematopoietic progenitors is also reported (3). The human ROBO4 cDNA encodes 1012 amino acids (aa), including a 27 aa signal sequence, a 440 aa extracellular domain (ECD) containing two C2‑type Ig domains and two fibronectin type III (FNIII) domains, a transmembrane domain and an intracellular domain. ROBO4 diverges from other ROBOs in the number of Ig, FNIII and cytoplasmic CC domains (1, 4). Within the ECD, human ROBO4 shares 80%, 80%, 87% and 88% aa identity with mouse, rat, bovine and canine ROBO4, respectively. Vascular endothelial ROBO4 is expressed at highest levels in during development and vascular remodeling, including tumor angiogenesis (1, 2, 4 ‑ 6). It is proposed to contribute to vascular stability. Consistent with this, endogenous ROBO4 is concentrated in the vascular stalk and sprouts rather than tip cells and appears to protect newly formed blood vessels against VEGF‑induced vascular leak (6 ‑ 9). ROBO4 binding of Slit proteins has been variably reported, and when detected may be mediated by ROBO4/ROBO1 heterodimers (2, 4 ‑ 7, 9 ‑ 13). ROBO4 is also variably reported to stimulate or inhibit cell migration or filopodia formation (2, 4 ‑ 13). Effects on cell movement may be mediated through intracellular binding of WASP‑, Ras/Rac/Rho‑, Mena‑, Src‑ or Paxillin‑related proteins, all of which affect the cytoskeleton (5 ‑ 7, 10 ‑ 12). Recombinant soluble ROBO4 ECD can antagonize endothelial cell migration and in vivo angiogenesis (13). 

References

  1. Huminiecki, L. et al. (2002) Genomics 79:547.
  2. Legg, J.A. et al. (2008) Angiogenesis 11:13.
  3. Shibata, F. et al. (2009) Stem Cells 27:183.
  4. Park, K.W. et al. (2003) Dev. Biol. 261:251.
  5. Seth, P. et al. (2005) Biochem. Biophys. Res. Commun. 332:533.
  6. Jones, C.A. et al. (2008) Nat. Med. 14:448.
  7. Jones, C.A. et al. (2009) Nat. Cell Biol. 11:1325.
  8. Chen, H. et al. (2010) Adv. Exp. Med. Biol. 664:457.
  9. London, N.R. et al. (2009) J. Thromb. Haemost. 7:57.
  10. Verissimo, A.R. et al. (2009) Biochem. Soc. Trans. 37:1214.
  11. Sheldon, H. et al. (2009) FASEB J. 23:513.
  12. Kaur, S. et al. (2008) BMC Cell Biol. 9:61.
  13. Suchting, S. et al. (2005) FASEB J. 19:121.

Long Name

Roundabout Homolog 4

Alternate Names

ECSM4, FLJ20798, Magic roundabout, MGC133352, MGC133353, MRB, roundabout homolog 4, roundabout homolog 4, magic roundabout (Drosophila)

Entrez Gene IDs

54538 (Human); 74144 (Mouse)

Gene Symbol

ROBO4

UniProt

Q8WZ75

Additional ROBO4 Products

Product Documents for Human ROBO4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ROBO4 Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.