Human ROBO4 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2454P

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 265703

View all ROBO4 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ROBO4
Gln28-Arg467
Accession # Q8WZ75

Specificity

Detects human ROBO4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant rat ROBO1, recombinant human (rh) ROBO2, or rhROBO3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human ROBO4 PE-conjugated Antibody

Detection of ROBO4 antibody in Human ROBO4 Transfectants antibody by Flow Cytometry.

Detection of ROBO4 in Human ROBO4 Transfectants by Flow Cytometry.

Human ROBO4 transfectants were stained with Mouse Anti-Human ROBO4 PE-conjugated Monoclonal Antibody (Catalog # FAB2454P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human ROBO4 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human ROBO4 transfectants

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ROBO4

ROBO4, also known as Magic Roundabout, is a ~150 kDa glycoprotein belonging to the ROBO family (1). ROBOs are molecular guidance receptors that typically interact with Slit ligands to regulate axon guidance and neuronal migration (2). Unlike other family members, ROBO4 is mainly restricted to the vascular endothelium (1, 2). Expression in early hematopoietic progenitors is also reported (3). The human ROBO4 cDNA encodes 1012 amino acids (aa), including a 27 aa signal sequence, a 440 aa extracellular domain (ECD) containing two C2-type Ig domains and two fibronectin type III (FNIII) domains, a transmembrane domain and an intracellular domain. ROBO4 diverges from other ROBOs in the number of Ig, FNIII and cytoplasmic CC domains (1, 4). Within the ECD, human ROBO4 shares 80%, 80%, 87% and 88% aa identity with mouse, rat, bovine and canine ROBO4, respectively. Vascular endothelial ROBO4 is expressed at highest levels during development and vascular remodeling, including tumor angiogenesis (1, 2, 4-6). It is proposed to contribute to vascular stability. Consistent with this, endogenous ROBO4 is concentrated in the vascular stalk and sprouts rather than tip cells and appears to protect newly formed blood vessels against VEGF-induced vascular leak (6-9). ROBO4 binding of Slit proteins has been variably reported, and when detected may be mediated by ROBO4/ROBO1 heterodimers (2, 4-7, 9-13). ROBO4 is also variably reported to stimulate or inhibit cell migration or filopodia formation (2, 4-13). Effects on cell movement may be mediated through intracellular binding of WASP-, Ras/Rac/Rho-, Mena-, Src- or Paxillin-related proteins, all of which affect the cytoskeleton (5-7, 10-12). Recombinant soluble ROBO4 ECD can antagonize endothelial cell migration and in vivo angiogenesis (13). 

References

  1. Huminiecki, L. et al. (2002) Genomics 79:547.
  2. Legg, J.A. et al. (2008) Angiogenesis 11:13.
  3. Shibata, F. et al. (2009) Stem Cells 27:183.
  4. Park, K.W. et al. (2003) Dev. Biol. 261:251.
  5. Seth, P. et al. (2005) Biochem. Biophys. Res. Commun. 332:533.
  6. Jones, C.A. et al. (2008) Nat. Med. 14:448.
  7. Jones, C.A. et al. (2009) Nat. Cell Biol. 11:1325.
  8. Chen, H. et al. (2010) Adv. Exp. Med. Biol. 664:457.
  9. London, N.R. et al. (2009) J. Thromb. Haemost. 7:57.
  10. Verissimo, A.R. et al. (2009) Biochem. Soc. Trans. 37:1214.
  11. Sheldon, H. et al. (2009) FASEB J. 23:513.
  12. Kaur, S. et al. (2008) BMC Cell Biol. 9:61.
  13. Suchting, S. et al. (2005) FASEB J. 19:121.

Long Name

Roundabout Homolog 4

Alternate Names

ECSM4, FLJ20798, Magic roundabout, MGC133352, MGC133353, MRB, roundabout homolog 4, roundabout homolog 4, magic roundabout (Drosophila)

Entrez Gene IDs

54538 (Human); 74144 (Mouse)

Gene Symbol

ROBO4

UniProt

Q8WZ75

Additional ROBO4 Products

Product Documents for Human ROBO4 PE-conjugated Antibody

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Product Specific Notices for Human ROBO4 PE-conjugated Antibody

For research use only

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