Human RUNX2/CBFA1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2006
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Transgenic Mouse
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human RUNX2/CBFA1 isoform 2
Lys219-Tyr404
Accession # Q13950
Lys219-Tyr404
Accession # Q13950
Specificity
Detects human RUNX2/CBFA1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) RUNX1 and rhRUNX3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human RUNX2/CBFA1 Antibody
Detection of RUNX2/CBFA1-regulated Genes by Chromatin Immunoprecipitation.
Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. RUNX2/CBFA1/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human RUNX2/CBFA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2006) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. TheVEGFpromoter was detected by standard PCR.Applications for Human RUNX2/CBFA1 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
Sample: PMA and calcium ionomycin treated Jurkat human acute T cell leukemia cell line chromatin, VEGF promoter detected by standard PCR.
Sample: PMA and calcium ionomycin treated Jurkat human acute T cell leukemia cell line chromatin, VEGF promoter detected by standard PCR.
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed MC3T3-E1 mouse preosteoblast cell line
Sample: Immersion fixed MC3T3-E1 mouse preosteoblast cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human RUNX2/CBFA1
Sample: Recombinant Human RUNX2/CBFA1
Reviewed Applications
Read 1 review rated 4 using AF2006 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RUNX2/CBFA1
Long Name
Runt-related Transcription Factor 2
Alternate Names
CBFA1
Entrez Gene IDs
860 (Human)
Gene Symbol
RUNX2
UniProt
Additional RUNX2/CBFA1 Products
Product Documents for Human RUNX2/CBFA1 Antibody
Product Specific Notices for Human RUNX2/CBFA1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...