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Human RUNX2/CBFA1 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF2006

R&D Systems, part of Bio-Techne
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BAF2006

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human RUNX2 isoform 2
Lys219-Tyr404
Accession # Q13950-2

Specificity

Detects human CBFA1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human RUNX2/CBFA1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human RUNX2/CBFA1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RUNX2/CBFA1

Core Binding Factor A1 (CBFA1; also called AML-3, PEBP-2 alphaA, OSF-2, RUNX2) is a 56 kDa DNA-binding protein that belongs to the Runt-related family of transcription factors. It is synthesized as a 521 amino acid (aa) protein with multiple domains. From the N-terminus, there is a 19 aa activation domain (AD1), a 41 aa glutamine/alanine (Q/A) repeat segment (AD2), a 128 aa Runt/DNA-binding region, a 9 aa NLS and a 285 aa ProSerThr (PST)-rich sequence that is needed for gene activation. Two isoforms exist, one with a truncated AD1 domain and another with a 22 aa deletion in the AD3 section of PST-rich sequence. Human CBFA1 is 98% aa identical to mouse CBFA1.

Long Name

Runt-related Transcription Factor 2

Alternate Names

CBFA1

Entrez Gene IDs

860 (Human)

Gene Symbol

RUNX2

UniProt

Additional RUNX2/CBFA1 Products

Product Documents for Human RUNX2/CBFA1 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human RUNX2/CBFA1 Biotinylated Antibody

For research use only

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