Human S100A16 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5180
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human S100A16
Ser2-Ser103
Accession # Q96FQ6
Ser2-Ser103
Accession # Q96FQ6
Specificity
Detects human S100A16 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 20% cross-reactivity with recombinant mouse S100A16 and less than 1% cross-reactivity with recombinant human (rh) S100A13 and rhS100B.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human S100A16 Antibody
S100A16 in Human Brain.
S100A16 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 10 µg/mL Sheep Anti-Human S100A16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5180) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human S100A16 by Western Blot.
Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human S100A16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5180) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A16 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human S100A16 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
1 µg/mL
Sample: Nalm-6 human Pre-B acute lymphocytic leukemia cell line
Sample: Nalm-6 human Pre-B acute lymphocytic leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A16
Long Name
S100 Calcium Binding Protein A16
Alternate Names
AAG13, DT1P1A7, S100F
Gene Symbol
S100A16
UniProt
Additional S100A16 Products
Product Documents for Human S100A16 Antibody
Product Specific Notices for Human S100A16 Antibody
For research use only
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