Human S100A9 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5578
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 474315
Product Specifications
Immunogen
E. coli-derived recombinant human S100A9
Met1-Pro114
Accession # P06702
Met1-Pro114
Accession # P06702
Specificity
Detects human S100A9 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) S100A1, 2, 7, 8, 11, 13, rhS100B, rhS100P, recombinant mouse S100A4, 6, 10, or 16 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human S100A9 Antibody
Detection of Human S100A9 by Western Blot.
Western blot shows lysates of human peripheral blood mononuclear cells (PBMC), human spleen tissue, human tonsil tissue, and human cartilage tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human S100A9 Monoclonal Antibody (Catalog # MAB5578) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
S100A9 in Capan-1 Human Cell Line.
S100A9 was detected in immersion fixed Capan-1 human pancreatic adenocarcinoma cell line using 10 µg/mL Mouse Anti-Human S100A9 Monoclonal Antibody (Catalog # MAB5578) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human S100A9 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed Capan-1 human pancreatic adenocarcinoma cell line
Sample: Immersion fixed Capan-1 human pancreatic adenocarcinoma cell line
Western Blot
0.5 µg/mL
Sample: Human peripheral blood mononuclear cells (PBMC), human spleen tissue, human tonsil tissue, and human cartilage tissue
Sample: Human peripheral blood mononuclear cells (PBMC), human spleen tissue, human tonsil tissue, and human cartilage tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A9
Human S100A9 (also MRP-14, Calgranulin-B, and p14) is a 14 kDa member of the S100 family of EF-hand calcium-binding proteins. It is 114 amino acids (aa) in length and contains short sequential modules. There is an N-terminal helical region, followed by a calcium-binding EF-hand domain, two more helical regions, a second EF-hand domain, and three additional helical regions. S100A9 will noncovalently heterodimerize with S100A8. In the presence of calcium, this heterodimer will form a heterotetramer. S100A9 is expressed in granulocytes, monocytes, and macrophages during acute and chronic inflammation. Human S100A9 shares 62% and 57% aa identity with rat and mouse S100A9, respectively.
Long Name
S100 Calcium Binding Protein A9
Alternate Names
Calgranulin B, MRP-14
Gene Symbol
S100A9
UniProt
Additional S100A9 Products
Product Documents for Human S100A9 Antibody
Product Specific Notices for Human S100A9 Antibody
For research use only
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