Human SCARA5 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB4900G

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 673527

View all SCARA5 Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human SCARA5
Arg83-His495
Accession # Q6ZMJ2

Specificity

Detects human SCARA5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse SCARA5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human SCARA5 Alexa Fluor® 488-conjugated Antibody

Detection of SCARA5 antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

Detection of SCARA5 in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human SCARA5 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB4900G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human SCARA5 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SCARA5

Scavenger Receptor A5 (SCARA5), also known as Tesr, is a 70-75 kDa kDa member of the scavenger receptor "supergroup" of molecules. It is one of at least six class A members that possess a collagen-like domain and form intramembrane homotrimers (1,3). Class A scavenger receptors are type II transmembrane proteins. Mature human SCARA5 consists of a 60 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 414 aa extracellular domain (ECD) with a spacer, a collagen-like, and a Scavenger Receptor Cysteine-Rich (SRCR) domain (3-6). Within the ECD, human SCARA5 shares 87% aa sequence identity with mouse and rat SCARA5. Among other class A members, it shares 23%‑34% aa sequence identity with CL-P1, MARCO, SCARA3, and SR-A1 in the ECD. Alternate splicing generates isoforms that lack the SRCR domain, the transmembrane segment, or the 225 aa spacer region between the membrane and the collagen-like domain (6). SCARA5 is a cell surface disulfide-linked homotrimer of 200-210 kDa (5,7). It is highly expressed by a number of cell types, including testicular Sertoli cells, transitional epithelium, respiratory pseudostratified columnar epithelium, intestinal M cells, astrocytes, retinal ganglion cells, endothelial cells and likely intestinal Paneth cells (5, 8). SCARA5 binds heat-killed bacterial particles but not yeast particles or modified LDL (5). It is also recognized to bind and transport L (light)-ferritin across anatomical boundaries (8). Finally, SCARA5 has also been found in both nucleus and cytoplasm (8).

References

  1. Martinez, V.G. et al. (2011) Pharmacol. Rev. 63:967.
  2. Mukhopadhyay, S. And S. Gordon (2004) Immunobiology 209:39.
  3. Zani, I.A. et al. (2015) Cells 4:178.
  4. Sarraj, M.A. et al. (2005) Dev. Dyn. 234:1026.
  5. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834.
  6. SwissProt Accession # Q6ZMJ2.
  7. Li, J.Y. et al. (2009) Dev. Cell 16:35.
  8. Mendes-Jorge, L. et al. (2014) PLoS One. 9:e106974.

Long Name

Scavenger Receptor Class A Member 5

Alternate Names

Tesr

Entrez Gene IDs

286133 (Human); 71145 (Mouse); 305974 (Rat)

Gene Symbol

SCARA5

UniProt

Q6ZMJ2

Additional SCARA5 Products

Product Documents

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Certificate of Analysis

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Product Specific Notices for Human SCARA5 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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