HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human SCARA5 Monoclonal Antibody (Catalog # MAB4900, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human SCARA5 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: HepG2 human hepatocellular carcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCARA5

Scavenger receptor A5 (SCARA5) is also known as testis expressed scavenger receptor (Tesr). Class A scavenger receptors, including CL-P1, MARCO, SCARA3, and SR-A1, are type II transmembrane proteins that contain a collagenous stalk (1-3). Mature human SCARA5 consists of a 60 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 414 aa extracellular domain (ECD) with a spacer, a collagen-like, and a scavenger receptor cysteine-rich (SRCR) domain (4-6). Within the ECD, human SCARA5 shares 87% aa sequence identity with mouse and rat SCARA5. It shares 23%‑34% aa sequence identity with CL-P1, MARCO, SCARA3, and SR-A1. Alternate splicing generates isoforms that lack the SRCR domain, the transmembrane segment, or the 225 aa spacer region between the membrane and the collagen-like domain (6). SCARA5 is a cell surface disulfide-linked homotrimer of > 250 kDa (5). It is highly expressed in testicular Sertoli and germ cells and more weakly in the epithelia of other tissues (4, 5). During mouse development, SCARA5 expression is attenuated in female embryos at the time of sex determination, whereas it is maintained in the developing testis (4). SCARA5 is re-expressed in the adult ovary (4). SCARA5 binds heat-killed bacterial particles but not yeast particles or modified LDL (5).

References

Murphy, J.E. et al. (2005) Atherosclerosis 182:1.
Mukhopadhyay, S. And S. Gordon (2004) Immunobiology 209:39.
Sarrias, M.R. et al. (2004) Crit. Rev. Immunol. 24:1.
Sarraj, M.A. et al. (2005) Dev. Dyn. 234:1026.
Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834.
Accession # Q6ZMJ2.

Long Name

Scavenger Receptor Class A Member 5

Alternate Names

Tesr

Entrez Gene IDs

286133 (Human); 71145 (Mouse); 305974 (Rat)

Gene Symbol

SCARA5

UniProt

Q6ZMJ2

Additional SCARA5 Products

Product Documents for Human SCARA5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SCARA5 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human SCARA5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4900

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all SCARA5 Antibodies »

Product Summary for Human SCARA5 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human SCARA5 Antibody

Detection of SCARA5 in HepG2 Human Cell Line by Flow Cytometry.