Human SCGF/CLEC11a Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB19041
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 239004
Product Specifications
Immunogen
E. coli-derived recombinant human SCGF/CLEC11a
Gly19-Phe245
Accession # BAA21499
Gly19-Phe245
Accession # BAA21499
Specificity
Detects human SCGF/CLEC11a in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human SCGF/CLEC11a Antibody
Detection of SCGF/CLEC11a in Lung Cancer.
SCGF/CLEC11a was detected in immersion fixed paraffin-embedded sections of Lung Cancer using Mouse Anti-Human SCGF/CLEC11a Monoclonal Antibody (Catalog # MAB19041) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human SCGF/CLEC11a Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Lung Cancer.
Sample: Immersion fixed paraffin-embedded sections of Lung Cancer.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SCGF/CLEC11a
Stem Cell Growth Factor (SCGF) is a secreted sulfated glycoprotein that belongs to the C-type lectin superfamily (CLEC11a) and is expressed primarily in hematopoietic tissues. It functions as a growth factor for primitive hematopoietic progenitor cells. SCGF-beta is a truncated variant of SCGF-alpha that has aa 78 amino acid deletion and lacks a functional calcium-dependent carbohydrate-recognition domain.
Long Name
Stem Cell Growth Factor/C-type Lectin Domain Family 11, Member A
Alternate Names
CLEC11a, CLECSF3, LSLCL
Gene Symbol
CLEC11A
UniProt
Additional SCGF/CLEC11a Products
Product Documents for Human SCGF/CLEC11a Antibody
Product Specific Notices for Human SCGF/CLEC11a Antibody
For research use only
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