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Human Serpin A4/Kallistatin Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1669

R&D Systems, part of Bio-Techne
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BAF1669

Key Product Details

Species Reactivity

Human

Applications

ELISA Detection (Matched Antibody Pair), Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Serpin A4/Kallistatin
Gln21-Pro427
Accession # AAA59454

Specificity

Detects human Serpin A4/Kallistatin in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross‑reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A5, rhSerpin C1, rhSerpin D1, rhSerpin E1, rhSerpin E2, and rhSerpin F2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Serpin A4/Kallistatin Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Serpin A4/Kallistatin (Catalog # 1669-PI)

Human Serpin A4/Kallistatin Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human Serpin A4/Kallistatin Antibody (Catalog # MAB16691)
  • Standard: Recombinant Human Serpin A4/Kallistatin Protein, CF (Catalog # 1669-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A4/Kallistatin

Serpin A4 is a member of the Serpin superfamily of the serine protease inhibitors (1). Known also as Kallistatin or proteinase inhibitor 4, Serpin A4 has been shown to function as a specific inhibitor of tissue kallikrein and as an inhibitor of angiogenesis and tumor growth (2, 3).

References

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Chen, V.C. et al. (2000) J. Biol. Chem. 275:40371.
  3. Miao, R.Q. et al. (2002) Blood 100:3245.

Alternate Names

Kallistatin

Entrez Gene IDs

5267 (Human)

Gene Symbol

SERPINA4

UniProt

Additional Serpin A4/Kallistatin Products

Product Documents for Human Serpin A4/Kallistatin Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Serpin A4/Kallistatin Biotinylated Antibody

For research use only

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