Human Siglec-8 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7975
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 837535
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec-8
Met17-Ala363
Accession # Q9NYZ4
Met17-Ala363
Accession # Q9NYZ4
Specificity
Detects human Siglec-8 in ELISAs. In direct ELISAs, no cross-reactivity
with recombinant human Siglec-7, -9, or -14 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Siglec-8 Antibody
Detection of Siglec-8 in Human Blood Granulocytes by Flow Cytometry.
Human peripheral blood granulocytes were stained with Rat Anti-Human CCR3 PE-conjugated Monoclonal Antibody (Catalog # FAB155P) and either (A) Mouse Anti-Human Siglec-8 Monoclonal Antibody (Catalog # MAB7975) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).Applications for Human Siglec-8 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood granulocytes
Sample: Human peripheral blood granulocytes
Reviewed Applications
Read 1 review rated 4 using MAB7975 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Siglec-8
CD33‑related SIGLEC family within the Ig superfamily of proteins. An alternately spliced 48 kDa short form lacks most of the intracellular domain, while another isoform lacks the second of three extracellular Ig domains, representing amino acids (aa) 152-245 of the 499 aa long form. Siglec-8 is expressed exclusively on eosinophils, mast cells, and weakly on basophils, and is a marker for eosinophil terminal differentiation. It specifically recognizes 6’ sulfated sialyl Lewis X carbohydrate structures. Engagement results in eosinophils apoptosis, or mast cell inhibition of degranulation. Eosinophil Siglec F is the mouse paralog of human Siglec-8, but shares <40% amino acid sequence identity.
Long Name
Sialic Acid Binding Ig-like Lectin 8
Alternate Names
SAF2, Siglec8, SIGLEC8L
Entrez Gene IDs
27181 (Human)
Gene Symbol
SIGLEC8
UniProt
Additional Siglec-8 Products
Product Documents for Human Siglec-8 Antibody
Product Specific Notices for Human Siglec-8 Antibody
For research use only
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