Human SIRP alpha/CD172a
Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB45462
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 602407
Product Summary
for Human SIRP alpha/CD172a
Antibody
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SIRP alpha/CD172a
Gly27-Asn370
Accession # P78324
Gly27-Asn370
Accession # P78324
Specificity
Detects human SIRP alpha/CD172a in ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human SIRP alpha/CD172a Antibody
Human SIRP alpha/CD172a ELISA Standard Curve.
Recombinant Human SIRP alpha/CD172a protein was serially diluted 2-fold and captured by Mouse Anti-Human SIRP alpha/CD172a Monoclonal Antibody (Catalog # MAB45462) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SIRP alpha/CD172a Monoclonal Antibody (Catalog # MAB45461) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human SIRP alpha/CD172a Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human SIRP alpha/CD172a Monoclonal Antibody (Catalog # MAB45461).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human SIRP alpha DuoSet ELISA (Catalog # DY4546-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SIRP alpha/CD172a
SIRP alpha (Signal regulatory protein alpha; also SHPS1 and BIT) is a variably glycosylated 90-120 kDa member of the SIRP family of proteins. It is widely expressed, being found on neurons, microglia/macrophages, endothelium, and fibroblasts. SIRP alpha has a variety of functions, including presynsptic organization, inhibition of integrin action, and induction of myogenesis. It binds to CD47 and likely other ligands. Mature human SIRP alpha is a 477 amino acid (aa) type I transmembrane glycoprotein. It contains an extracellular region (aa 27-372) that shows one V-type Ig-like (aa 32-137) and two C2-type Ig-like domains (aa 147-347). Its cytoplasmic domain possesses two ITIMs which interact with protein tyrosine phosphatases. There is one alternative start site at Met102 plus a four aa insertion after Gln421. Over aa 27-370, human SIRP alpha shares 61% aa identity with mouse SIRP alpha.
Long Name
Signal-regulatory Protein alpha
Alternate Names
BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA
Entrez Gene IDs
Gene Symbol
SIRPA
UniProt
Additional SIRP alpha/CD172a Products
Product Documents for Human SIRP alpha/CD172a Antibody
Product Specific Notices for Human SIRP alpha/CD172a Antibody
For research use only
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