Human Sirtuin 5/SIRT5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5914
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Sirtuin 5/SIRT5
Met33-Ser310
Accession # Q9NXA8
Met33-Ser310
Accession # Q9NXA8
Specificity
Detects human Sirtuin 5/SIRT5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SIRT1, rhSIRT2, and rhSIRT6 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Sirtuin 5/SIRT5 Antibody
Detection of Human Sirtuin 5/SIRT5 by Western Blot.
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Sirtuin 5/SIRT5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5914) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Sirtuin 5/SIRT5 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Sirtuin 5/SIRT5 in Human Liver.
Sirtuin 5/SIRT5 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Sirtuin 5/SIRT5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5914) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Sirtuin 5/SIRT5 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Sirtuin 5/SIRT5, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Sirtuin 5/SIRT5, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sirtuin 5/SIRT5
Long Name
Silent Mating Type Information Regulation 5 Homolog
Alternate Names
SIR2L5, SIRT5
Gene Symbol
SIRT5
UniProt
Additional Sirtuin 5/SIRT5 Products
Product Documents for Human Sirtuin 5/SIRT5 Antibody
Product Specific Notices for Human Sirtuin 5/SIRT5 Antibody
For research use only
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