Human SLPI Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1274
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Rat, Bacteria - Stenotrophomonas maltophilia
Applications
Validated:
Immunohistochemistry, Neutralization, Western Blot
Cited:
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Mass Spectrometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SLPI
Specificity
Detects human SLPI in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with mouse SLPI is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human SLPI Antibody
SLPI in Human Lung.
SLPI was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human SLPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1274) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human SLPI Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung
Sample: Immersion fixed paraffin-embedded sections of human lung
Western Blot
0.1 µg/mL
Sample: Recombinant Human SLPI (Catalog # 1274-PI)
Sample: Recombinant Human SLPI (Catalog # 1274-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SLPI
References
- Zhang, D. et al. (2002) J. Biol. Chem. 277:29999.
- Taggart, C.C. et al. (2002) J. Biol. Chem. 277:33648.
Long Name
Secretory Leukocyte Protease Inhibitor
Alternate Names
ALK1, ALPProtease inhibitor WAP4, antileukoproteinase, BLPISeminal proteinase inhibitor, HUSI, HUSI-I, Mucus proteinase inhibitor, secretory leukocyte peptidase inhibitor, Secretory leukocyte protease inhibitor, secretory leukocyte protease inhibitor (antileukoproteinase), WAP four-disulfide core domain protein 4, WAP4HUSI-1, WFDC4MPI
Gene Symbol
SLPI
Additional SLPI Products
Product Documents for Human SLPI Antibody
Product Specific Notices for Human SLPI Antibody
For research use only
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