Smad4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Smad4 Monoclonal Antibody (Catalog # MAB2097) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Smad4 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cell line was stained with Mouse Anti-Human Smad4 Monoclonal Antibody (Catalog # MAB2097, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human Smad4 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: HeLa human cell line fixed with paraformaldehyde and permeabilized with saponin

Reviewed Applications

Read 1 review rated 5 using MAB2097 in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Smad4

Mothers Against Decapentaplegic homolog 4 (Smad4) belongs to a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta) superfamily signals from the cell surface to the nucleus. Upon signal-induced phosphorylation, Smad subunits associate with the common-mediator subunit, Smad4. This heteromeric complex then translocates into the nucleus to exert transcriptional comodulator activity.

Long Name

Mothers Against DPP Homolog 4

Alternate Names

DPC4, MADH4

Entrez Gene IDs

4089 (Human)

Gene Symbol

SMAD4

UniProt

Q13485

Additional Smad4 Products

Product Documents for Human Smad4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Smad4 Antibody

This product is covered by one or more of the following US patents: 5,712,097; 5,814,457; 5,955,292 and other pending US patent applications and foreign equivalents.

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Smad4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2097

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Smad4 Antibody

Smad4 in HeLa Human Cell Line.