Human Smoothened Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10276

R&D Systems, part of Bio-Techne
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MAB10276-100
MAB10276-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1015418

View all Smoothened Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human Smoothened
Met1-Tyr233
Accession # Q99835

Specificity

Detects human Smoothened in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Smoothened Antibody

Smoothened antibody in Human Liver Cancer Tissue by Immunohistochemistry (IHC-P).

Smoothened in Human Liver Cancer Tissue.

Smoothened was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human Smoothened Monoclonal Antibody (Catalog # MAB10276) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Smoothened Antibody

Application
Recommended Usage

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Smoothened

Smoothened homolog (SMO) is a G protein-coupled receptor that is a component of the hedgehog signaling pathway. SMO can function as an oncogene. Activating SMO mutations can lead to unregulated activation of the hedgehog pathway and serve as driving mutations for cancers such as medulloblastoma, basal-cell carcinoma, pancreatic cancer, and prostate cancer. As such, SMO is an attractive cancer drug target, along with the many hedgehog pathway agonists and antagonists that are known to directly target SMO.

Alternate Names

bnb, CRJS, FZD11, Gx, SMO, SMOH

Entrez Gene IDs

6608 (Human); 319757 (Mouse); 25273 (Rat)

Gene Symbol

SMO

UniProt

Q99835

Additional Smoothened Products

Product Documents for Human Smoothened Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Smoothened Antibody

For research use only

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