Human SMPD1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5348
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SMPD1
His62-Pro628
Accession # NP_000534
His62-Pro628
Accession # NP_000534
Specificity
Detects human SMPD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse SMPD1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human SMPD1 Antibody
Detection of Human SMPD1 by Western Blot.
Western blot shows lysates of human cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SMPD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5348) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SMPD1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human SMPD1 by Simple WesternTM.
Simple Western lane view shows lysates of human brain (cerebellum), loaded at 0.2 mg/mL. A specific band was detected for SMPD1 at approximately 87 kDa (as indicated) using 50 µg/mL of Goat Anti-Human SMPD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5348) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human SMPD1 Antibody
Application
Recommended Usage
Simple Western
50 µg/mL
Sample: Human brain (cerebellum)
Sample: Human brain (cerebellum)
Western Blot
1 µg/mL
Sample: Human cerebellum tissue
Sample: Human cerebellum tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SMPD1
Sphingomyelin phosphodiesterase, also known as acid sphingomyelinase and encoded by the SMPD1 gene, is a lysosomal phosphodiesterase which belongs to the acid sphingomyelinase family (1). SMPD1 catalyzes the hydrolysis of sphingomyelin to ceramide and phosphorylcholine. Ceramide, a bioactive lipid, has emerged as an important signaling molecule involved in a variety of cellular processes such as cell differentiation, apoptosis, and proliferation (2). Activation of SMPD1 occurs by the removal, chemical modification or dimerization of its C-terminal cysteine residue (3). Deficiencies of SMPD1 result in a lysosomal storage disorder referred to as Niemann-Pick disease (4). rhSMPD1 was expressed without the last three C-terminal residues, and is therefore constitutively active.
References
- Schuchman, E.H. et al. (1991) J. Biol. Chem. 266:8531.
- Melendez, A.J. et al. (2008) Biochim. Biophys. Acta 1784:66.
- Qiu, H. et al. (2003) J. Biol. Chem. 278:32744.
- Smith, E.L. and Schuchman, E.H. (2008) FASEB J. 22:3419.
Long Name
Sphingomyelin Phosphodiesterase 1, Acid Lysosomal
Alternate Names
aSMase, NPD
Gene Symbol
SMPD1
UniProt
Additional SMPD1 Products
Product Documents for Human SMPD1 Antibody
Product Specific Notices for Human SMPD1 Antibody
For research use only
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