Human SOX17 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1924
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry
Cited:
Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # 245013
Product Specifications
Immunogen
E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2
Asp177-Val414
Accession # Q9H6I2
Specificity
Detects human SOX17 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human SOX2, 3, 7, or 10.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Scientific Data Images for Human SOX17 Antibody
SOX17 in B16 Mouse Cell Line.
SOX17 was detected in immersion fixed B16 mouse melanoma cell line using Mouse Anti-Human SOX17 Monoclonal Antibody (Catalog # MAB1924) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human SOX17 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human embryonic stem cell derived endodermal progenitors and B16 mouse melanoma cell line
Sample: Immersion fixed human embryonic stem cell derived endodermal progenitors and B16 mouse melanoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB1924 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX17
References
- Kanai-Azuma, M. et al. (2002) Development 129:2367.
- Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
Long Name
Transcription Factor SOX17
Alternate Names
FLJ22252, SRY (sex determining region Y)-box 17, SRY-related HMG-box transcription factor SOX17, transcription factor SOX-17, VUR3
Entrez Gene IDs
64321 (Human)
Gene Symbol
SOX17
UniProt
Additional SOX17 Products
Product Documents for Human SOX17 Antibody
Product Specific Notices for Human SOX17 Antibody
For research use only
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